The Forgotten And Misunderstood pKa Of Buffering Systems In IVF Media
Have you observed the following? Let’s say you’re using TCM199, or another 25 mM bicarbonate buffered medium containing phenol red as the pH indicator. As soon as you remove the medium from the 5-6% CO2 incubator, you begin to see whisps of magenta-colouring within the medium. In a matter of minutes, the medium has changed […]
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