In Vitro Fertilisation (IVF) is a reproductive technology utilised to accelerate genetic gain within a herd. Market analysis shows that the IVF technology is rapidly being adopted, with predominant markets in South America and North America. Data from International Embryo Transfer Society (IETS) 2023 report has demonstrated significant growth of the IVF or In Vitro […]
What is IVF? The Benefits and Disadvantages… Read More »
Have you observed the following? Let’s say you’re using TCM199, or another 25 mM bicarbonate buffered medium containing phenol red as the pH indicator. As soon as you remove the medium from the 5-6% CO2 incubator, you begin to see whisps of magenta-colouring within the medium. In a matter of minutes, the medium has changed
The Forgotten And Misunderstood pKa Of Buffering Systems In IVF Media Read More »
Improving and standardising in vitro fertilisation (IVF) techniques offers numerous benefits, drives innovation and enhances productivity. Standardisation ensures that IVF procedures are consistent, reliable, and reproducible across different settings, leading to uniform outcomes. This is particularly important for large-scale agricultural operations where consistency in livestock and embryo quality is essential. Enhanced IVF techniques can lead
Mastering Embryology Techniques for Bovine Gamete and Embryo Successful Outcomes Read More »
Written by Founder, Prof. Jeremy Thompson. As the Founder of ART Lab Solutions and a dedicated researcher in the field of cattle embryo production (IVP) for the past 45 years, I have witnessed remarkable advancements. It is gratifying to see IVP become the preferred method for disseminating high-value female genetics from OPU-collected oocytes and for
You have decided to set-up your own cattle IVF lab, and you have gone to a bovine IVF course (view our services page for more information on training). In any IVF lab, the quality control aspect is critical to success, but how can you measure if your lab is functioning well? Enter the ‘Bovine Embryo
Quality Control with Abattoir-Derived Oocytes: The Bovine Embryo Assay Read More »
Regular maintenance of your laboratory and it’s equipment is important. Maintenance includes calibrating equipment regularly (daily if possible otherwise a couple times a week), keeping track of batches of media, culture oils and laboratory consumables being used and being aware of potential sources of volatile organic compounds (VOCs). While some of this tracking can seem
Mastering Laboratory Techniques for Bovine Gamete and Embryo Successful Outcomes Read More »
Embryo production media is a delicate commodity that demands refrigeration to maintain its effectiveness. Factors like exposure to light (specifically radiation) and oxygen, in conjunction with the media’s specific composition, determine its shelf life. Notably, media with intricate formulations are at a heightened risk of undergoing oxidative deterioration as time goes by. One crucial
Storing Media: Understanding Its Shelf Life Read More »
Evaluating embryo quality prior to embryo transfer or cryopreservation relies on an embryologist’s experience. Within commercial laboratories, embryo quality is assessed visually using a dissecting microscope. Grading usually takes into account several factors including cell numbers, size of the inner cell mass, developmental stage compared to embryo age, the presence of extruded or degenerated cells,
Grading cattle embryos for transfer or cryopreservation Read More »
The oocytes have been fertilised, and we now have presumptive zygotes! How can we give these zygotes the best opportunity to develop into competent blastocysts, ready to be cryopreserved or transferred into recipient cattle? Here are some of our tips following IVF: 1. Remove cumulus cells quickly and thoroughly to reduce the metabolic requirements on
Tips following IVF. Read More »
The most accurate way to measure the fertilisation ability of semen in vitro is to test it using abattoir-derived cumulus-oocyte-complexes (COCs).
The Importance of Testing Semen Prior to Use During In Vitro Fertilisation (IVF) Read More »
Looks like you haven't made a choice yet.
