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Defining Success in Commercial Bovine IVF Labs: A Closer Look

The Repro Group, based in Queensland, Australia have successfully been accelerating genetic performance in livestock through using assisted reproductive technologies.   They have been successfuly using ART Lab Solutions media and vitrification solutions since 2021. One of their more recent results included a live calf rate of 70% from pregnancy with A grade embryos. These […]

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Juvenile In Vitro Fertilisation Embryo Transfer (JIVET)

Written by Dr Jen Kelly. The ability to utilise oocytes from juvenile animals for accelerated genetic gain and rapid multiplication of animals has significant implications for livestock improvement programs.  Image reprinted with kind permission, JM Kelly However there is great variation in response and oocyte developmental capability both between and within animals.      Age

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Marie Ellul

New Product Release – Transport VitroMat Protect

We have developed a new product called Transport VitroMat Protect, a zwitterionic buffered maturation media not requiring controlled CO2  gassing. This newly created product is supplemented with a specific endoplasmic reticulum stress inhibitor that improves blastocyst development rates compared to existing options on the market.   To ensure convenient availability, we have made the product

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What does “serum-free” medium really mean?

While scanning the cattle IVF literature, you will likely see the references that a medium, especially culture medium, is “serum-free”. As we have already discussed in previous blogs, addition of serum is a ‘two-edged sword’. On the one-hand it can promote development, particularly if embryo growth is slightly retarded and morula formation is hindered. But on the other-hand, serum addition at levels of 5% or more will increase triglyceride content that negatively impacts cryo-survival, especially with ‘slow-freezing’ protocols. Furthermore, serum addition is a causal factor for ‘Large Offspring Syndrome’.

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Embryo culture drop size – is there an optimum?

We recently had an excellent question from one of our clients, who wanted to know if our recommendation of 5 micro-litres (uL) of medium per embryo cultured was critical or not, or could it be changed to suit their IVP embryo production system. Firstly, our protocol recommendations are based on our long-history of research and commercial application with the media formulations we have, and this is why we provide our preferred protocol.

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The Buzz On Growth Factors In Embryo Culture Medium

Back in the ‘80’s -‘90’s when cattle IVF began to be seriously used for breeding, and when the “large calf syndrome” was recognised as a consequence of embryo culture, the use of growth factors in medium was snubbed, as it was thought that such factors caused the syndrome. The real truth is that some cytokines and growth factors are very supportive of embryo development and quality. They decrease embryo ‘stress’, and reduce the incidence of cellular apoptosis (regulated cell death).

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