Embryo production media is a delicate commodity that demands refrigeration to maintain its effectiveness. Factors like exposure to light (specifically radiation) and oxygen, in conjunction with the media’s specific composition, determine its shelf life. Notably, media with intricate formulations are at a heightened risk of undergoing oxidative deterioration as time goes by.
One crucial step in preserving the longevity of bottled media is limiting its exposure to air, and thereby, oxygen. Techniques like vacuum-sealing and using inert gases like nitrogen can significantly extend the storage life of media post-production.
However, a question often arises: once a bottle is uncapped and comes into contact with the environment (even when refrigerated between 4 – 8°C), what is its effective duration? Our comprehensive research indicates that our serum-free medium retains its efficacy for about two weeks post-opening. Hence, we strongly advocate for its consumption within this period.
But, what about the duration for which media can be kept in storage? The answer lies in the degradation rates of its individual constituents, inherently linked to its composition. It’s universally accepted that fresher media yields more reliable outcomes. To combat the inconsistencies that arise from prolonged storage, some choose to incorporate serum or its alternatives. This addition not only enhances media’s stability but also mitigates some of the decomposition effects over its lifespan. Based on our analysis, we can confidently assert a 4-month shelf life for our unopened media preserved under nitrogen.