The most accurate way to measure the fertilisation ability of semen in vitro is to test it using abattoir-derived cumulus-oocyte-complexes (COCs). While often sperm has been assessed for motility and morphology when collected and cryopreserved, sometimes internal issues (eg. DNA fragmentation) that cannot be visualised by microscopy can severely impact IVF results.

These results can then be used by producers to finalise in vitro mating’s of commercially collected COC/oocytes from their valuable donor cows and heifers. It will also assist in the preparation of enough semen straws per bull at the time of IVF.

Taking the time to test semen using abattoir-derived COCs prior to commercial use in vitro could ultimately result in economic benefits for the service provider. Embryo production rates, and more importantly, pregnancy and calving outcomes from these oocytes will also be maximised.